phospho (p)–s6k1 t389 antibody  (Cell Signaling Technology Inc)

Journal: World Journal of Acupuncture - Moxibustion

Article Title: Effects of electroacupuncture on central insulin signal transduction-related proteins in insulin resistance model rats via the mTORC1/S6K1 pathway

doi: 10.1016/j.wjam.2025.06.007

Figure Lengend Snippet: Fig. 2. Effects of electroacupuncture on mTORC1 and S6K1 and their phosphorylated protein expression in IR rats (mean ± SD, 10 rats/group). Notess: a P < 0.05,compared with the N group; b P < 0.05,compared with the M group; c P < 0.05,compared with the EA group; d P < 0.05,compared with the L + EA group.

Article Snippet: This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) oster Biological Technology, BM0627, BA1051, BA1054), rabbit olyclonal antibodies for insulin receptor substrate-1(IRS-1), p-IRS, protein kinase B (Akt), p-Akt, glycogen synthase kinase-3 β(GSKβ), p-GSK-3 β , S6K1, p-S6K1 (Affinity, AF6273, AF3273, AF6261, F0016, AF5016, AF2016), rabbit monoclonal mammalian target of apamycin (mTOR), p-mTOR (Abcam, Ab32028, Ab109268), HRPonjugated goat anti-mouse and anti-rabbit secondary antibodies, y3-labeled goat anti-rabbit IgG, concentrated normal goat serum blocking) (Wuhan Boster Biological Technology, BA1051, BA1054, R1009, BA1032), PMSF (Aladdin, P105539), RIPA lysis buffer, BCA rotein assay kit, DAPI (Beyotime, P0 013B, P0 010, C1002), TEMED Sinopharm Chemical Reagent Co., Ltd., 80125336), and protein arker (Helix, P12103–2).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Effects of varying valine concentrations on the relative expression levels of downstream genes in the mTOR signaling pathway. ( A – E ) represent the relative mRNA expression levels of the five key downstream targets of the mTOR pathway: EIF4EBP1 , EIF4E , S6K1 , EEF2 , and RPTOR . The error bars represent the SD ( n = 3). ANOVA was used to detect the differences between groups, and the LSD method was combined for uncorrected exploratory pairwise comparisons, while the Duncan method was used for conservative multiple comparison corrections. Different lowercase letters indicate significant differences ( p < 0.05), while different uppercase letters indicate significant differences ( p < 0.01).

Article Snippet: , P-S6K1 , affbiotech , AF3228 , 5% egg white , 1:1000.

Techniques: Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Effects of different valine concentrations on the phosphorylation of downstream proteins in the mTOR signaling pathway. ( A ) Western blotting was used to detect the protein expression levels of phosphorylated mTOR (P-mTOR) and phosphorylated S6K1 (P-S6K1). ( B ) The phosphorylation level of mTOR protein (P-mTOR/mTOR) was quantified and calculated using β-actin and mTOR as internal references. ( C ) The phosphorylation level of S6K1 protein (P-S6K1/S6K1) was quantified and calculated using β-actin and S6K1 as internal references. ( D ) Western blotting was used to detect the protein expression levels of phosphorylated 4EBP1 (P-4EBP1) and phosphorylated RPS6 (P-RPS6). ( E ) The phosphorylation level of 4EBP1 protein (P-4EBP1/4EBP1) was quantified and calculated using β-actin and 4EBP1 as internal references. ( F ) The phosphorylation level of RPS6 protein (P-RPS6/RPS6) was quantified and calculated using β-actin and RPS6 as internal references. ( G ) Western blotting was used to detect the protein expression levels of phosphorylated eEF2 (P-eEF2). ( H ) The phosphorylation level of eEF2 protein (P-eEF2/eEF2) was quantified and calculated using β-actin and eEF2 as internal references. The error bars represent the SD ( n = 3). ANOVA was used to detect the differences between groups, and the LSD method was combined for uncorrected exploratory pairwise comparisons, while the Duncan method was used for conservative multiple comparison corrections. Different lowercase letters indicate significant differences ( p < 0.05), while different uppercase letters indicate significant differences ( p < 0.01).

Article Snippet: , P-S6K1 , affbiotech , AF3228 , 5% egg white , 1:1000.

Techniques: Phospho-proteomics, Western Blot, Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Effects of valine and rapamycin on the phosphorylation levels of mTOR downstream proteins. ( A ) The phosphorylation levels of mTOR (P-mTOR) in MAC-T cells treated with varying concentrations of rapamycin were assessed using Western blotting. ( B ) The P-mTOR level (P-mTOR/mTOR) in MAC-T cells treated with 100 nM rapamycin was quantified and calculated using β-actin and mTOR as internal references. ( C ) Western blotting was used to detect the protein expression levels of P-mTOR and P-S6K1. ( D ) The P-mTOR level (P-mTOR/mTOR) was quantified and calculated using β-actin and mTOR as internal references. ( E ) The P-S6K1 level (P-S6K1/S6K1) was quantified and calculated using β-actin and S6K1 as internal references. ( F ) Western blotting was used to detect the protein expression levels of P-4EBP1 and P-RPS6. ( G ) The P-4EBP1 level (P-4EBP1/4EBP1) was quantified and calculated using β-actin and 4EBP1 as internal references. ( H ) The P-RPS6 level (P-RPS6/RPS6) was quantified and calculated using β-actin and RPS6 as internal references. ( I ) Western blotting was used to detect the protein expression levels of P-eEF2 and α-casein. ( J ) The P-eEF2 level (P-eEF2/eEF2) was quantified and calculated using β-actin and eEF2 as internal references. ( K ) The α-casein protein expression level was quantified using β-actin as an internal reference. The error bars represent the SD ( n = 3). ANOVA was used to detect the differences between groups, and the LSD method was combined for uncorrected exploratory pairwise comparisons, while the Duncan method was used for conservative multiple comparison corrections. Different lowercase letters indicate significant differences ( p < 0.05), while different uppercase letters indicate significant differences ( p < 0.01).

Article Snippet: , P-S6K1 , affbiotech , AF3228 , 5% egg white , 1:1000.

Techniques: Phospho-proteomics, Western Blot, Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Primer sequences of internal reference genes and target genes.

Article Snippet: , P-S6K1 , affbiotech , AF3228 , 5% egg white , 1:1000.

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Valine on the Synthesis of α-Casein in MAC-T Cells and the Expression and Phosphorylation of Genes Related to the mTOR Signaling Pathway

doi: 10.3390/ijms26073179

Figure Lengend Snippet: Detailed information of antibodies.

Article Snippet: , P-S6K1 , affbiotech , AF3228 , 5% egg white , 1:1000.

Techniques:

Journal: Cell reports

Article Title: The Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex ubiquitinates Rheb and supports amino acid-induced mTORC1 activation

doi: 10.1016/j.celrep.2024.115101

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-p-S6K1 T389 , Cell Signaling Technology , Cat#9205; RRID:AB_330944.

Techniques: Virus, Recombinant, Magnetic Beads, Control, Negative Control, Ubiquitin Proteomics, shRNA, Plasmid Preparation, Immunofluorescence, Software